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gdna eraser  (TaKaRa)


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    Structured Review

    TaKaRa gdna eraser
    Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+fast+rt+reagent+kit/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/pmc13091134-83-17-20
    Average 99 stars, based on 426 article reviews
    gdna eraser - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Fibro-Adipogenic Progenitors Regulate Orofacial Neuromuscular Junction Regeneration via Myostatin.
    Article Snippet: RNA was extracted from cultured cells using TRIzol (Invitrogen, USA) according to the manufacturer's protocol. .. RNA was reverse transcribed into cDNA using the PrimeScript FAST RT reagent Kit with gDNA Eraser (Takara, Japan). .. Quantitative real- time PCR was performed using the TB Green Premix Ex Taq II (Tli RNaseH Plus, Takara, Japan) on a LightCycler480 system (Roche Diagnostics, USA).

    Article Title: Engineering a Tyrosol-Responsive Dynamic System in Saccharomyces cerevisiae for Adaptive Regulation of Hydroxytyrosol Biosynthesis.
    Article Snippet: Static metabolic regulation during microbial synthesis of polyphenolic compounds leads to tyrosol accumulation, inducing cellular toxicity and growth inhibition.. Dynamic regulation effectively alleviates the imbalance between cell growth and production; yet, a tyrosol-responsive dynamic system for yeast adaptive regulation remains unreported.. In this study, endogenous tyrosol-responsive promoters were mined via transcriptomics, and the optimal promoter PYOR153W was identified.

    Article Title: C -mannosyl tryptophan dynamics in a mouse model of the peritoneal dissemination of ovarian cancer
    Article Snippet: Total RNA was extracted with TRizol solution (Life Technologies Japan Ltd) according to the manufacturer’s protocol. .. The quantity and quality of isolated RNA were confirmed spectrophotometrically, and total RNA was reverse transcribed using the PrimeScript FAST RT reagent kit with a gDNA eraser (Takara Bio Inc.). .. Target DNA was amplified by Brilliant III Ultra-Fast SYBR Green QPCR Master Mixes (Agilent) with specific primers (listed in ) using complementary DNA as a template, and the amplified signal was monitored by Thermal Cycler Dice Real Time System IV (Takara Bio Inc.).

    Article Title: Maternal immune activation causes sex-specific impairment of microglial function during prefrontal cortex development.
    Article Snippet: Gestational maternal immune activation (MIA) has been recognized as a risk factor for neurodevelopmental disorders (NDDs) later in life.. Furthermore, sex is a significant modifying factor for NDDs.. Microglia, the resident immune cells of the brain, appear to be critical mediators of MIA-associated NDD pathology and are known to play a pivotal role in the pathological process of MIA-induced behavioral abnormalities.

    Article Title: Osmotic signaling governs sunscreen biosynthesis to safeguard desert cyanobacteria against desiccation
    Article Snippet: .. Total RNA was extracted using Trizol Reagent (Cwbio), and reverse transcription (RT) of RNA samples was performed using the PrimeScript FAST RT Reagent Kit (TaKaRa) following DNA Figure 7. ..

    Article Title: Deletion of D345L gene attenuates ASFV and Induces Protection against Homologous and Heterologous challenge by enhancing host innate immunity.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, USA). .. Reverse transcription was performed using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara, Dalian, China). .. RT-qPCR was conducted with TB Green (Takara, Dalian, China).

    Synthesized:

    Article Title: Characterization of a Splice Variant in FLNA Associated With Periventricular Nodular Heterotopia
    Article Snippet: .. Residual genomic DNA was removed, and cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (RR092A, TaKaRa) according to the manufacturer's instructions. .. PCR amplification of cDNA was performed with PrimerSTAR MAX DNA Polymerase (R045A, TaKaRa).

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury
    Article Snippet: Total RNA was extracted from cells using the Eastep Super Total RNA Extraction Kit (LS1040, Shanghai Promega, China) according to the manufacturer's protocol. .. First-strand cDNA was then synthesized from the extracted RNA using the PrimeScript Fast RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Quantitative PCR was subsequently performed using TB Green Premix Ex Taq II Fast (CN830A, Takara, Japan) on a QuantStudio 5 real-time PCR system (Applied Biosystems, USA).

    Isolation:

    Article Title: C -mannosyl tryptophan dynamics in a mouse model of the peritoneal dissemination of ovarian cancer
    Article Snippet: Total RNA was extracted with TRizol solution (Life Technologies Japan Ltd) according to the manufacturer’s protocol. .. The quantity and quality of isolated RNA were confirmed spectrophotometrically, and total RNA was reverse transcribed using the PrimeScript FAST RT reagent kit with a gDNA eraser (Takara Bio Inc.). .. Target DNA was amplified by Brilliant III Ultra-Fast SYBR Green QPCR Master Mixes (Agilent) with specific primers (listed in ) using complementary DNA as a template, and the amplified signal was monitored by Thermal Cycler Dice Real Time System IV (Takara Bio Inc.).



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